Figure 1.

CYCH;1 expression is down-regulated by drought stress and CYCH;1 is localized to the nucleus. A, qRT-PCR analysis of the time course of CYCH;1 expression in wild-type plants after treatment with drought, ABA, mannitol, and NaCl. Error bars represent the se of three independent experiments. B, Histochemical GUS staining of PCYCH;1:GUS transgenic plants. GUS staining of a 12-d-old seedling (top) of a flower (middle, left) and silique (middle, right) of a 5-week-old plant and of the epidermal layer of a mature rosette leaf of a 3-week-old plant (bottom). Bar = 60 μm. C, Subcellular localization of GFP:CYCH;1. A nuclear localization signal (NLS) fused with red fluorescent protein (RFP) was cotransfected into protoplasts with GFP:CYCH;1. Bars = 10 μm.