Table IV. Novel splicing events.

The column labeled Majority Pollen Supported reports the number of new splicing events where 80% (four of five) or more of the supporting gapped reads were from the pollen RNA-Seq data set. Internal refers to new junctions that are internal to currently annotated gene models and thus represent likely alternative splicing or cases where the annotated gene model is inaccurate. Extended refers to new junctions that extend 5′ or 3′ of the currently annotated gene models and in most cases represent new UTR exons. NA, Not applicable.

Minimum Read Support	New Splicing Events		Majority Pollen Supported	Genes
	Internal	Extended
1	17,527	1,045	NA	8,547
2	9,407	597	NA	5,601
5	3,821	276	998	2,757
10	1,908	153	550	1,518
20	872	74	303	724
50	287	29	128	259