Table II. Proteins identified as involved in important metabolism in Nannochloropsis spp. from day 10 to day 31 during long-term nitrate starvation.
Ratio represents fold change in relative abundance. Ratio greater than 1.5-fold means that a protein is increased significantly (P < 0.05). Ratio less than or equal to 1.5-fold means that a protein is reduced significantly (P < 0.05). Values are based on three biological replicates. Further information regarding the protein identification is accessible at Supplemental Table S3 and https://www.box.com/s/q5oy0thdhlvda29vclq2.
| Spot No. | Protein Name | Unique Peptides Matched with MS/MS Spectra | Mascot Protein Score | Ratio, Day 16/Day 10 | Ratio, Day 31/Day 10 |
|---|---|---|---|---|---|
| Photosynthesis and carbon fixation | |||||
| 1 | CbbX protein homolog | 7 | 563 | −2.0 | −1.8 |
| 2 | Chloroplast FNR | 6 | 672 | −0.8 | −2.9 |
| 3 | Chloroplast FNR | 4 | 257 | −0.8 | −1.5 |
| 4 | Ribulose-phosphate 3-epimerase | 3 | 407 | −1.2 | −1.4 |
| 5 | Light-harvesting complex protein | 6 | 526 | −1.1 | −1.7 |
| 6 | Light-harvesting complex protein | 4 | 445 | −1.2 | −1.5 |
| 7 | Light-harvesting complex protein | 5 | 541 | 1.0 | −1.5 |
| 22 | Light-harvesting complex protein | 3 | 293 | −1.0 | −2.5 |
| 8 | Violaxanthin/Chl a-binding protein precursor | 2 | 186 | 0.8 | −1.5 |
| 9 | Oxygen-evolving enhancer protein | 2 | 310 | −0.6 | −2.7 |
| 10 | Carbonic anhydrase | 1 | 116 | −1.3 | −2.7 |
| Nitrogen assimilation and urea cycle | |||||
| 11 | Gln synthetase | 9 | 887 | 3.0 | 2.1 |
| 12 | Argininosuccinate synthetase | 2 | 319 | 1.7 | 1.4 |
| 13 | Gln synthetase | 8 | 1100 | 1.8 | 2.5 |
| H+ transport and endocytosis | |||||
| 14 | V-type H-ATPase | 2 | 139 | 1.7 | 0.9 |
| 15 | Vacuolar proton pump | 13 | 1290 | 1.7 | 2.0 |
| 16 | Receptor-mediated endocytosis protein | 1 | 89 | 1.3 | 1.7 |
| Antioxidant | |||||
| 17 | Cytochrome c peroxidase | 3 | 382 | 1.4 | 1.0 |
| 18 | l-Ascorbate peroxidase | 4 | 452 | −0.8 | 0.8 |
| 19 | 2-Cys peroxiredoxin | 4 | 516 | 1.9 | |
| Protein metabolism | |||||
| 20 | Proteasome subunit α | 4 | 562 | 1.0 | 2.1 |
| 21 | Translation elongation factor EF-Ts | 4 | 377 | −1.4 | −4.2 |
| Fatty acid metabolism, glycolysis, and TCA cycle | |||||
| 23 | Enoyl-ACP reductase | 2 | 315 | 3.6 | 4.0 |
| 24 | Trans-2-enoyl-CoA reductase | 5 | 471 | 2.0 | 2.2 |
| 25 | Stearoyl-ACP desaturase | 8 | 691 | −1.2 | −1.9 |
| 26 | 1,3-β-Glucanase | 8 | 708 | 1.2 | 1.5 |
| 27 | PGK | 4 | 490 | 0.8 | 1.5 |
| 28 | PK | 7 | 725 | 1.0 | 1.2 |
| 29 | PPH | 5 | 807 | 0.7 | 1.0 |
| 30 | GAPDH | 5 | 589 | 1.0 | 1.5 |
| 32 | Enoyl-ACP reductase | 2 | 218 | −1.3 | −2.6 |
| 33 | β-Chain succinyl-CoA synthetase | 8 | 952 | 1.6 | 1.1 |