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eCA kinetics. A and B, Effects of assay pH on eCA activity in T. pseudonana (Tp; A) and T. weissflogii (Tw; B). C and D, Reaction rates (CO2 hydration rate) as a function of substrate concentration (CO2) in T. pseudonana (C) and T. weissflogii (D). A Michaelis-Menten fit to the T. weissflogii data, which shows some saturation, gives a CO2 half-saturation constant of 87 ± 10 μm and a maximal reaction rate of 26 ± 2 × 10−14 mol cell−1 s−1.
