Figure 3.
E2F-regulated expression of ASF1A and ASF1B genes. A, Expression levels of ASF1A, ASF1B, and CDC6 genes determined by qRT-PCR analysis in transgenic plants overexpressing each of the six Arabidopsis E2F transcription factors (E2FOE). Measurements are relative to the amount in wild-type (WT) plants. Asterisks indicate statistical differences applying Student’s t test (P < 0.05). B, Relative expression levels of ASF1A, ASF1B, and CDC6 genes determined by qRT-PCR analysis in transgenic plants overexpressing RepA protein compared with plants overexpressing RepA-E198K protein. C, ChIP assays using anti-myc or anti-HA antibodies with nuclei prepared from wild-type or transgenic plants expressing myc-E2Fd, HA-E2Fe, or HA-E2Ff. The immunoprecipitates and input DNA before immunoprecipitation were analyzed by PCR for the presence of promoter sequences of ASF1A and ASF1B; ACT2, a control gene that is not regulated by E2F factors; and MCM3, a control gene that is regulated by E2F factors. Three PCR experiments were done with each sample.