Fatty acid analysis (A–F) and fatty acyl-CoA profiling (G). A to F, Fatty acids were extracted from lyophilized leaves (A), stems (B), aerial parts (C) and roots (D) of young seedlings, flowers (E), and silique walls (F) of the wild type (Col-0), kcs9, and complementation lines (com1, com11, and com12) and analyzed using GC. G, Fatty acyl-CoA profiling was performed on lyophilized roots of wild-type and kcs9 mutant plants. The x axis represents the carbon chain length of the fatty acids. Values shown are means of four experiments ± sd. Asterisks denote statistical differences with respect to the wild type: *P < 0.05, **P < 0.01.