Table II. Fatty acid profiles of leaf total fatty acid (L), leaf TAG (L-TAG), root total fatty acid (R), and root TAG (R-TAG) from material harvested 35 DAS.
Values represent mean percentages of total fatty acids ± sd from separate analysis (n = 6 for total fatty acid profiling in both leaves and roots, n = 4 for TAG profiling in both leaves and roots). Parentheses show P values for statistical significance between the transgenic line and the wild type calculated by Student’s two-tailed t test. nd, Not detected; ns, not significant.
| Construct | C16:0 | C16:1 | C16:3 | C18:0 | C18:1 | C18:2 | C18:3 | C20:1 | C22:0 | C24:0 | C24:1 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Wild type (L) | 17.2 ± 0.9 | 4 ± 0.2 | 11.3 ± 0.9 | nd | 1.6 ± 0.1 | 11.8 ± 1.1 | 54.2 ± 4.2 | nd | nd | nd | nd |
| Vector control (L) | 16.6 ± 0.4 (ns) | 3.6 ± 0.1 (P = 0.0126) | 11.5 ± 0.5 (ns) | nd | 1.5 ± 0.1 (ns) | 12.2 ± 0.3 (ns) | 54.6 ± 1.3 (ns) | nd | nd | nd | nd |
| D1 (L) | 16.4 ± 1.6 (ns) | 3.5 ± 0.6 (ns) | 11.5 ± 1.6 (ns) | nd | 1.6 ± 0.4 (ns) | 12.6 ± 1.9 (ns) | 54.4 ± 7 (ns) | nd | nd | nd | nd |
| D1o0-0 (L) | 17 ± 1.3 (ns) | 3.5 ± 0.4 (P = 0.0173) | 11.8 ± 1.2 (ns) | nd | 1.5 ± 0.3 (ns) | 11.2 ± 0.8 (ns) | 54.6 ± 5.5 (ns) | nd | nd | nd | nd |
| D1o1-1 (L) | 16.8 ± 0.7 (ns) | 3.5 ± 0.4 (ns) | 13.3 ± 1 (P = 0.0106) | nd | 4.6 ± 0.4 (P = 3.1 E-09) | 12.5 ± 1.1 (ns) | 49.2 ± 3.1 (P = 0.0255) | nd | nd | nd | nd |
| D1o1-3 (L) | 15.7 ± 1.9 (ns) | 3.1 ± 0.4 (P = 0.0011) | 10.9 ± 1.5 (ns) | nd | 4.1 ± 0.3 (P = 1.5 E-09) | 11.6 ± 2.4 (ns) | 54.5 ± 6.7 (ns) | nd | nd | nd | nd |
| D1o3-1 (L) | 14.8 ± 0.5 (P = 0.0002) | 3.1 ± 0.1 (P = 1.1 E-05) | 11 ± 0.9 (ns) | nd | 4 ± 0.2 (P = 1.9 E-10) | 19.7 ± 1.4 (P = 1.04 E-06) | 47.4 ± 4.8 (P = 0.0197) | nd | nd | nd | nd |
| D1o3-3 (L) | 14 ± 0.3 (P = 6.2 E-06) | 2.1 ± 0.2 (P = 1.3 E-08) | 8.8 ± 0.3 (P = 6.4 E-05) | nd | 12.2 ± 0.5 (P = 2.7 E-13) | 29 ± 0.3 (P = 6.2 E-12) | 32.4 ± 0.9 (P = 1.8 E-07) | 0.2 ± 0.04 | 0.3 ± 0.07 | 0.5 ± 0.02 | 0.4 ± 0.03 |
| Wild type (R) | 22.8 ± 0.5 | 0.3 ± 0.02 | nd | 1 ± 0.07 | 2.6 ± 0.2 | 39.2 ± 1.3 | 28.5 ± 1.6 | nd | 3.3 ± 0.3 | 2.5 ± 0.2 | nd |
| D1o3-3 (R) | 15.7 ± 0.3 (P = 2.0 E-11) | 0.4 ± 0.1 (ns) | nd | 2.8 ± 0.1 (P = 9.1 E-11) | 12.8 ± 0.6 (P = 6 E-11) | 45.9 ± 1 (P = 1.5 E-06) | 17.8 ± 0.7 (P = 3.2 E-08) | nd | 2.5 ± 0.1 (P = 9.8 E-05) | 1.6 ± 0.1 (P = 8.5 E-07) | 0.5 ± 0.1 |
| Wild type (L-TAG) | 37.0 ± 3.4 | nd | nd | nd | 8.8 ± 2.2 | 23.7 ± 5.1 | 30.5 ± 1.4 | nd | nd | nd | nd |
| D1o3-3 (L-TAG) | 12.5 ± 0.05 (P = 7.5 E-06) | 0.9 ± 0.01 | 1.6 ± 0.03 | 1.6 ± 0.01 | 14.5 ± 0.01 (P = 0.0022) | 35.6 ± 0.2 (P = 0.0035) | 29.7 ± 0.09 (P = 0.2936) | 0.7 ± 0.07 | 0.8 ± 0.02 | 0.8 ± 0.1 | 1.2 ± 0.08 |
| Wild type (R-TAG) | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd |
| D1o3-3 (R-TAG) | 15.1 ± 0.1 | 0.7 ± 0.02 | nd | 5.7 ± 0.2 | 21.6 ± 0.2 | 37.1 ± 0.4 | 14.1 ± 0.2 | 0.3 ± 0.03 | 3.1 ± 0.1 | 1.4 ± 0.1 | 0.8 ± 0.02 |