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Activity of SbHCT mutants. Mutation of the active-site residues identified in the crystal structure results in the reduction of SbHCT kinetic activity. A, Michaelis-Menten kinetics for the wild type (WT) and S38A and Y40A mutants of SbHCT. B, Relative specific activities of mutants compared with that of wild-type SbHCT. The activities were measured at 750 µm shikimate and compared with the wild type in percentage terms.
