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Immunoblot analysis of the seed protein from glup6 lines using anti-GLUP6/GEF antibody. The arrowhead indicates the GLUP6/GEF protein-specific band (53.8 kD). Note that EM939, EM1327, and EM1484 are devoid of GLUP6/GEF protein, indicating that premature termination of translation caused by the nonsense mutation results in general instability of the truncated GEF polypeptide or nonsense-mediated decay of the transcript. WT, Wild type.
