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. 2013 Apr 11;162(2):663–674. doi: 10.1104/pp.113.217869

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Figure 7. — In vitro GEF assay of GLUP6/GEF. The conformational changes of Rab5s and Rab11 (as 1 μm GST fusion proteins) upon GDP/guanylyl imidodiphosphate exchange were measured by monitoring Trp autofluorescence when incubated with 0 μm (blue), 0.25 μm (magenta), 0.5 μm (yellow), and 1 μm (red) GST-GLUP6/GEF. GLUP4/Rab5 and GLUP4/Rab5 (Gly-45Asp) indicate the wild type and mutant of rice GLUP4/Rab5 in EM960, respectively. Note that GST-GLUP6/GEF activates GLUP4/Rab5 as well as the Arabidopsis Rab5s, ARA6 and ARA7, but not Arabidopsis Rab11.