Effects of altered EBE expression on plant phenotype. A, EBE up-regulation causes an increase and its down-regulation causes a decrease of the leaf initiation rate as compared with the wild type (WT). Threads of the following colors were used for labeling rosette leaves: orange for leaves L1 and L2, yellow for leaves L3 and L4, and white for leaves L5 and L6. B, Number of rosette leaves of transgenic and wild-type plants during a period of 4 weeks. The symbols represent means ± sd of 11 plants at each time point. At 30 d after sowing (DAS), there is a statistically significant difference between the wild type and all transgenic plants (Student’s t test after false discovery rate correction, P < 0.05). C, Altered phyllotaxy and SAM size in transgenic plants compared with the wild type. The left panel shows an enlarged SAM in a 35S:EBE plant (OX-11) that had arrested without developing further. The right panel shows an arrested SAM in the EBE-RNAi-50 line that occurred after cotyledon development. The three middle panels show the changed phyllotaxy of transgenic plants in comparison with the wild type. In some of the 35S:EBE plants, the angle between the two first true leaves was only about 120°, while in EBE-RNAi plants, the angle was more than 150°C.