Figure 1.

Expression pattern of OsHMA2 gene. A, Tissue-dependent expression of OsHMA2 at vegetative stage. Plants were grown in a nutrient solution in the absence of zinc, iron, manganese, or copper or presence of these metals for 7 d. The roots, shoots, and basal region (2 cm from the root-shoot junction) were sampled for expression analysis. B, Response of OsHMA2 to excess zinc (10 µm) and cadmium (1 µm) for 1 d in vegetative roots. C, Spatial expression in seminal root. Different segments (0–1 cm and 1–2 cm from the apex) were sampled from 5-d-old seedlings. D, Tissue-specific expression at flowering stage. Different tissues samples were taken from rice grown in a paddy field at flowering stage. The expression was determined by quantitative RT-PCR, and expression level of Actin and HistoneH3 were used as an internal control. Error bars represent ± sd of three independent biological replicates. Statistical comparison was performed by one-way ANOVA followed by the Tukey’s multiple comparison test (A and B) or Student’s t test (C). Differences were not significant between different metal deficiency treatment and the control condition (A and B); **P < 0.01 (C).