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The wxr3 mutation does not directly affect SCFTIR1/AFB-dependent auxin signaling. A, GUS staining of pHS:AXR3NT-GUS in the wxr3 mutant background at different time points with or without IAA treatment after 2 h at 37°C temperature treatment. B, GUS activity determined by MUG assay of the seedlings in A. C, Levels of Aux/IAA and GH3 transcripts in wild-type (WT) and wxr3 seedlings treated with 20 μm IAA for 1 h. Error bars represent se.
