Figure 6.

The wxr3 mutation does not affect the stability of PIN proteins on the plasma membrane. A, RNA levels of AUX1 and PINs in wild-type (WT) and wxr3 seedlings as measured by quantitative RT-PCR. Error bars represent se. B, Disappearance of proteins from the plasma membrane after treatment with 50 μm CHX is not affected by the wxr3 mutation. Roots were treated with antibody against PIN1/PIN2 (left) or PM-ATPase (right). PIN1 and PM-ATPase levels were quantified and are expressed as arbitrary units over time.