Table I. Sequences bound by group IXa ERFs in vitro.
Prefixes to gene names indicate organisms (tobacco [Nt] and periwinkle [Cr]). Positions of 5′ ends of the indicated sequences relative to the first ATG and orientations (forward [F] and reverse [R]) of boxes in the context of promoters are indicated to the left of the sequences. TESS scores are indicated as follows: a, based on data from ERF189 at the P box; b, ORCA3 at the P box; c, ORCA3 at the CS1; d, ORCA3 at the GCC box, and e, AtERF1 at the GCC box. Sequences of 7 bp that give rise to the scores in d and e are in the rightmost column. Nd, Not detected.
| Name | Gene | Sequence (5′ to 3′) |
TESS Scores |
Sequence for Scores d and e | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| a | b | c | d | e | |||||||
| P type | |||||||||||
| P | NtPMT2 | −133 F | CCGCCCTCCA | 7.45 | 11.56 | nd | 4.39 | 3.42 | (CGCCCTC) | ||
| Q1 | NtQPT2 | −131 R | TAGCACTCCA | 5.58 | 10.96 | nd | nd | nd | |||
| Q2 | NtQPT2 | −160 F | AAGCACTCCA | 7.18 | 10.69 | nd | nd | nd | |||
| Q3 | NtQPT2 | −238 R | TAGCACTCCA | 5.58 | 10.96 | nd | nd | nd | |||
| O3 | NtODC1 | −969 R | TAGCCAGCCT | 7.45 | 8.92 | nd | 3.02 | nd | (GCCAGCC) | ||
| O5 | NtODC2 | −919 R | TAGCCAGCCT | 7.45 | 8.92 | nd | 3.02 | nd | (GCCAGCC) | ||
| M1 | NtMATE1 | −206 R | TAGCACTCCA | 7.75 | 7.44 | nd | 2.99 | 2.89 | (AGCACCC) | ||
| M2 | NtMATE1 | −704 R | GAGCACACCT | 9.04 | 9.27 | nd | nd | nd | |||
| CC3 | CrCPR | −346 R | ACGCCTACCA | 3.58 | 8.79 | nd | nd | nd | |||
| Consensus | AAA | ||||||||||
| N/GC/NNCC/ | |||||||||||
| CCC | |||||||||||
| CS1 type | |||||||||||
| CS1 | CrSTR | −146 F | TAGACCGCCT | 6.20 | nd | 9.11 | 5.85 | 7.29 | (GACCGCC) | ||
| CC2 | CrCPR | −342 R | AAGAACGCCT | 7.36 | nd | 12.36 | 2.54 | 4.73 | (GAACGCC) | ||
| CC1 | CrCPR | −224 F | ACGCCGGCGA | nd | 7.28 | nd | 6.16 | 4.97 | (CGCCGGC) | ||
| GCC | |||||||||||
| GCC | NtGLN2 | −1179 F | AGAGCCGCCA | nd | nd | nd | 10.29 | 10.50 | (AGCCGCC) | ||