Figure 10.

Expression of AtERF4 and AtERF8 during leaf senescence of wild-type (WT) and quintuple tcp plants. A, The delayed yellowing of leaves in quintuple tcp mutant. The sixth leaf of wild-type (top) and quintuple tcp (bottom) plants of different ages was photographed. B, Chlorophyll content in the sixth leaf of the wild type and quintuple tcp mutant at indicated ages. The error bars and asterisks indicate sd (n = 12) and sds at P < 0.001 by Student’s t test. C, Expression of SAG12, AtERF4, and AtERF8 in the wild type and the quintuple tcp mutant. Transcript levels were determined with RT-PCR using aliquots of total RNAs from the sixth leaves of the wild type (black squares) and quintuple tcp mutants (white triangles) at the indicated ages. Each value of 3-week-old leaves was set at 1. Error bars indicate sd of technical triplicates.