Figure 2.

Low accumulation of NtERF3 in plant cells. A, GFP florescence in the nucleus of transgenic tobacco cells in which the individual fused gene was introduced (left). 4′,6-Diamidino-2-phenylindole staining (right) shows the location of the nucleus. B, Immunoblot analysis of transgenic tobacco cells after introduction of the fusion genes. Two independent lines per construct were subjected to immunoblot analysis using an anti-GFP antibody. The molecular size markers are shown on the left. C, GFP florescence of Pro-35S:NLS-GFP-NtERF3 and Pro-35S:NLS-GFP-NtERF3 (∆83/190) roots of 3-d-old Arabidopsis seedlings. NLS, Nuclear localization signal. Bars = 10 µm (A) and 50 µm (C).