Figure 5.

Ability of leucine rotamer library to inhibit Vpr dimerization measured by BiFC signal.

Note: (A) Small molecules (leucine rotamers) were screened for their ability to interfere with Vpr dimerization measured by BiFC fluorescence using high content imaging. Cells transfected with Venus-Vpr plasmids were treated with leucine rotamer library (1 μM) or vehicle control (DMSO) and assessed for BiFC signal. Data was normalized to vehicle exposed transfected cells using Z-score methods. The average and standard deviation from two independent experiments were calculated and plotted. (B) The average number of viable cells per field, after excluding cells for morphology and nuclear size, were recorded for each well (based on the average of >20 fields per well) using the image software. Figure represents one of two independent experiments.

Abbreviations: Vpr, viral protein R; BiFC, bimolecular fluorescence complementation; TF, transfection; PBS, phosphate buffered saline; DMSO, dimethyl sulfoxide.