Skip to main content
. Author manuscript; available in PMC: 2013 May 31.
Published in final edited form as: FEBS Lett. 2012 Mar 6;586(7):1004–1008. doi: 10.1016/j.febslet.2012.02.028

Table 1.

Apparent Km and Vmax of Staphylococcus aureus and Bacillus subtilis BshA. For L-malate concentration dependence, 10 mM UDP-N-acetylglucosamine (UDP-GlcNAc) fixed and L-malate concentration varied from 0.1mM to 3.2 mM; for UDP-GlcNAc concentration dependence, 3 mM of L-malate fixed and UDP-GlcNAc varied from 1 mM to 15 mM; and, 0.35 μg BshA added to the reactions.

Substrate Km [μM] Vmax [nmol min-1mg-1]
L-malate
S. aureus 710 ± 110 35 600 ± 1800
B. subtilis 410 ± 70 11 200 ± 100
UDP-GlcNAc
S. aureus 250 ± 110 34 200 ± 3700
B. subtilis 370 ± 94 8700 ± 100