Table 2.

Substrate specificity of S. aureus and B. subtilis BshA. For B. subtilis BshA, reaction conditions consisted of 0.3 mM acceptor substrate, 1.0 mM UDP-GlcNAc and 0.16 μg BshA. For S. aureus BshA, reaction conditions consisted of 0.7 mM acceptor substrate, 3.0 mM UDP-GlcNAc and 0.048 μg BshA.

	S. aureus		B. subtilis
Substrate	Specific Activity (nmoles min-1 mg-1)	Relative Rate (%)	Specific Activity (nmoles min-1 mg-1)	Relative Rate (%)
L-malic acid	18,600 ± 6,300	100	6,600 ± 150	100
D-malic acid	<100	<0.5	230 ± 8	3.4
inositol-1-L-phosphate	<100	<0.5	<100	<0.5
glycolic acid	<100	<0.5	<100	<0.5
D-lactic acid	<100	<0.5	<100	<0.5
D, L-isocitric acid	<100	<0.5	<100	<0.5
D-glyceric acid	430 ± 180	2.3	<100	<0.5
citric acid	<100	<0.5	<100	<0.5