Fig. 2.

DNA gel electrophoresis of various Chaetomium samples. a DNA electrophoresis in 1 % agarose of RT-PCR products isolated from Ch. globosum grown under standard conditions (lane 1, PD medium with no oxidative stress) or oxidative stress induced by addition of 0.1 mM paraquat (lane 2), or 0.05 M peroxyacetic acid (lane 3), or 5 mg/mL Cd²⁺ (lane 4), or 10 mM H₂O₂ (lane 5). In lanes 1–5, the same reaction volume of RT-PCR product from Ch. globosum was loaded for comparison. Products isolated from Ch. cochliodes grown under standard conditions (lane 6) or oxidative stress induced by addition of 0.1 mM paraquat (lane 7) or 0.05 M peroxyacetic acid (lane 8), or 5 mg/ml Cd²⁺ (lane 9), or 10 mM H₂O₂ (lane 10). In lanes 6–10, the same reaction volume of RT-PCR product was loaded. For all samples loaded in lanes 1–10, the combination of primers CgkatGNterFWD & CgkatGintREV2 was used for amplification (Table 1). B) DNA electrophoresis in 1 % agarose of control RT-PCR products from Ch. cochliodes (lanes 11–13) and Ch. globosum (lanes 14–16). The same reaction volume of RT-PCR product was loaded in lanes 11–16 in different growth phases: lanes 11 and 14 after 1 day of cultivation, lanes 12 and 15 after 2 days of cultivation and lanes 13 and 16 after 3 days of cultivation; For all samples loaded in lanes 11–16 the combination of primers CgTIMfwd & CgTIMrev was used for amplification (Table 1). Lanes M molecular mass standards with size given in base pairs (bp)