Figure 4.

Nuclear localization of Sty1 is not sufficient to induce promoter recruitment in the absence of Sty1 activation.

(A) ChIP assays to assess the recruitment of Sty1 to stress-induced promoters in caf1Δ cells. Assays were carried out as in Figure 3A using samples prepared from sty1-myc caf1Δ and wild type cells that had been exposed to 1M sorbitol for 15 minutes.

(B) ChIP assays as in (A) but using cells that had been exposed to 2mM H₂O₂ for 15 minutes.

(C) Results of indirect immunofluoresence to assess the nuclear localization of Sty1 in caf1Δ cells in the absence and presence of stress (2mM H₂O₂ for 15 minutes). More than 500 cells were counted for each strain in each condition. The results of three independent experiments are shown.