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. 2013 May 22;14:11. doi: 10.1186/1471-2199-14-11

Figure 5.

Figure 5

SDS-PAGE and Western blot analyses of recombinant His-Stra8 proteins and total proteins extracted fromS. meridionalis gonads. a) Stra8 protein with His-tag expressed in E. coli and total proteins extracted from 50 dah ovaries and 130 dah testes of S. meridionalis were analyzed by SDS-PAGE followed by Coomassie blue staining. b) The endogenous mature peptide of Stra8 was further demonstrated with Stra8 polyclonal antibody by chemiluminescence.