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. 2013 May 16;3:12. doi: 10.1186/2044-5040-3-12

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Copyright © 2013 Strömberg et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Satellite cell containing two X chromosomes. Immunofluorescent staining visualized by fluorescent microscopy shows XY-FISH combined with staining for the SC marker CD56. The section was examined with a 100× objective. (A,B) CD56 (green), together with laminin-1 (red), with and without DAPI. The CD56 positive satellite cell is located just beneath the basal lamina of the muscle fiber. (C,D) The same SC after XY-FISH, with and without DAPI, showing the presence of two X chromosomes (bright green, arrows).