Table 3.

Citrus grapefruit leaf proteins that were up-accumulated in response to Las-infection

Spota	ASVb	Protein function/namec	Accession #	Theoreticald		Se	Mf	Eg
	UP IP US IS			Mr	pI
		Energy/Metabolisms
29i		Granule-bound starch synthase	gi|223029784	67320	8.56
33i		Granule-bound starch synthase	gi|223029784	67320	8.56
61i		Granule-bound starch synthase	gi|223029784	67320	8.56
		Redox homeostasis
34i		Peroxiredoxins, prx-1, prx-2, prx-3, putative	gi|255578581	29299	8.38
119i		Cu/Zn superoxide dismutase	gi|2274917	12784	5.82
147i		2Fe-2S ferredoxin-like protein	gi|18397961	17602	7.75
		Pathogen response
14i		Chloroplastic light/drought-induced stress protein	gi|22261807	35216	5.24
15i		Acidic class I chitinase	gi|23496445	34123	4.70
19		Acidic class I chitinase	gi|23496445	36735	4.81	64	8	24
39		Lectin-related protein precursor	gi|11596188	29272	5.10	72	7	32
41h		Chitinase	gi|1220144	32459	5.06	53	5	20
43		Chitinase	gi|1220144	36735	4.81	73	11	41
44		Lectin-related protein precursor	gi|11596188	29272	5.10	85	7	32
66i		Chitinase	gi|1220144	31909	5.06
88i		Chitinase	gi|1220144	31909	5.06
92i		Chitinase	gi|1220144	31909	5.06
124		Chitinase	gi|1220144	36735	4.81	110	11	45
141i		CAP160 protein	gi|22327778	65970	5.07
153		Putative miraculin-like protein 2	gi|119367468	17806	6.74	105	7	49
156i		Miraculin-like protein 2	gi|11596180	25652	6.10
160i		Chitinase	gi|1220144	31909	5.06
179i		PR-4 type protein	gi|3511147	15227	5.50
181i		Miraculin-like protein 2	gi|87299377	24120	5.61
187i		Miraculin-like protein 2	gi|87299377	24120	5.61
202		Putative miraculin-like protein 2	gi|119367468	23610	8.18	126	9	54
203i		Miraculin-like protein 2	gi|87299377	24120	5.61

^a The spot numbers correspond to the numbers given in Figure  2 and Additional file 3: Figure S2.

^b Average spot volume per treatment group; UP, uninfected reference for pre-symptomatic plants; IP, infected pre-symptomatic plants; US, uninfected reference for symptomatic plants; IS, infected symptomatic plants. Average spot volumes separated by letters to show significant difference are presented in Additional file 6: Appendix S1.

^c Protein function/name was determined by http://www.ncbi.nlm.nih.gov/BLAST/.

^d Theoretical relative molecular weight (M_r) and isoelectric point (pI) were calculated by http://www.expasy.org/. Observed M_r and pI can be extrapolated from Figure  1.

^e Mascot score of protein hit.

^f Number of matched peptide masses. The sequences of PMF-matched peptides per spot are provided in Additional file 7: Appendix S2.

^g Percent sequence coverage of matched peptides.

^h Protein identification confirmed by MALDI-TOF-MS/MS. Sequencing information is provided in Additional file 7: Appendix S2.

ⁱ Protein identification confirmed by LC-MS/MS.