Table 2.
Differential accumulated primary metabolites in heat treated pericarp compared to control pericarp
| Sugars | 2 h | 1 d | 6 d | 32 d |
|---|---|---|---|---|
| Turanose |
1.19 * |
1.13 |
1.36 * |
1.25 * |
| Galactose |
1.44 * |
1.59 * |
1.09 |
1.17 |
| Fructose |
1.29 * |
1.36 * |
1.02 |
1.08 |
| Glucose |
1.4 * |
1.51 * |
1.06 |
1.14 |
| Sucrose |
1.21 * |
1.09 |
1.07 |
1.19 |
| 4-Keto-glucose |
1.59 * |
1.09 |
1.43 * |
0.84 |
| Arabinose |
1.36 * |
0.71 * |
1.23 * |
0.18 * |
| Mannose |
1.06 |
1.11 |
0.88 |
0.73 * |
| Xylose |
0.82 * |
1.37 * |
0.82 * |
0.98 |
| Glucopyranose |
0.81 * |
2.09 * |
0.84 |
1.41 * |
|
Organic acids |
2 h |
1 d |
6 d |
32 d |
| Pentonic acid |
1.77 * |
0.78 * |
1.04 |
1.46 * |
| Succinic acid |
1.13 |
1.2 * |
1.26 * |
1.54 * |
| Hydroxypyruvic acid |
1.16 |
1.07 |
1.28 * |
0.71 * |
| 2-Ketoglutaric acid |
1.06 |
1.1 |
0.65 * |
1.31 * |
| 2-Keto-d-gluconic acid |
1.05 |
1.56 * |
1.13 |
2.52 * |
| Gluconic acid |
1 |
1.81 * |
0.92 |
1.04 |
| Isocitric acid |
0.85 |
2.1 * |
1.11 |
1.15 |
| 2,3,4-Trihydroxybutyric acid |
0.84 |
1.67 * |
0.61 * |
1.2 * |
| Ethanedioic acid |
0.83 * |
1.26 * |
1.11 |
1.55 * |
| Citric acid |
0.83 * |
1.53 * |
1.22 * |
1.92 * |
| Malic acid |
0.63 * |
1 |
0.77 * |
1.41 * |
| 4-N-Trimethylsilylmethylaminobutyric acid |
0.56 * |
1.03 |
0.76 * |
1.33 * |
| GABA |
0.41 * |
1.23 * |
0.76 * |
1.25 * |
|
Amino acids |
2 h |
1 d |
6 d |
32 d |
| Ornithine |
2.63 * |
3.69 * |
2.34 * |
2.87 * |
| Valine |
0.48 * |
0.99 |
0.46 * |
0.7 * |
| 5-oxo-L-proline |
0.45 * |
1.08 |
0.73 * |
1.14 |
| Glycine |
0.37 * |
1.42 * |
0.65 * |
1.44 * |
| Alanine |
0.31 * |
2.22 * |
0.69 * |
1.25 * |
| Threonine |
0.29 * |
1.12 |
0.47 * |
0.92 |
| Aspartic acid |
0.14 * |
0.99 |
0.35 * |
0.8 * |
| L-proline |
0.13 * |
0.99 |
0.19 * |
0.65 * |
| Serine |
0.13 * |
1.27 * |
0.2 * |
0.64 * |
| Glutamine |
0.11 * |
0.86 |
0.3 * |
0.73 * |
| Asparagine |
0.03 * |
0.34 * |
0.25 * |
0.89 |
|
Alcohols |
2 h |
1 d |
6 d |
32 d |
| Glycerol |
1.61 * |
1.2 * |
0.91 |
2.23 * |
| Arabitol |
1.05 |
1.04 |
0.39 * |
0.92 |
| Rhamnitol |
0.98 |
0.97 |
0.76 * |
1.73 * |
| Sorbitol |
0.94 |
0.68 * |
1.87 * |
1.8 * |
|
Fatty acids |
2 h |
1 d |
6 d |
32 d |
| Oleic acid |
UP * |
UP * |
UP * |
UP * |
| Tetradecanoic acid |
UP * |
UP * |
UP * |
UP * |
| Octadecanoic acid |
0.65 * |
1.11 |
0.94 |
1.66 * |
| 9,12-Octadecadienoic acid |
0.57 * |
0.84 |
1.05 |
0.93 |
| Hexadecanoic acid |
0.62 * |
1.06 |
0.99 |
1.38 * |
| Hexadecanoic acid,2,3-bisoxypropylester |
0.68 * |
0.39 * |
0.81 * |
2.18 * |
|
Others |
2 h |
1 d |
6 d |
32 d |
| Phosphate | 0.89 | 1.48 * | 1.33 * | 1.48 * |
Samples at 2 h after treatment and 1, 6 and 32 d after harvest were used for the differential primary metabolic profiling analysis. A 300 mg sample was extracted in 2,700 μl of methanol. Extracts were determined with GC-MS used ribitol as internal standard. A total of 45 metabolites were differentially accumulated in heat treated pericarp. This table shows the ratio (HT/control during the same period of storage). *: significant difference (P <0.05). UP: metabolite was detected in the heat treated pericarp but not in the control pericarp.