Table 3.
Secondary metabolites specifically accumulated in heat treated pericarp
| Secondary metabolites | 2 h | 1 d | 6 d | 32 d |
|---|---|---|---|---|
| Quercetin-dihexose-deoxyhexose |
16.71 |
14.81 |
15.42 |
n.s. |
| Hesperetin |
12.32 |
2.08 |
n.s. |
n.s. |
| Naringenin chalcone-hexose |
15.04 |
n.s. |
4.77 |
n.s. |
| Vanillic acid |
14.73 |
n.s. |
n.s. |
14.37 |
| Hydroxybenzoic acid-hexose |
14.99 |
n.s. |
n.s. |
n.s. |
| Diosmin |
4.06 |
n.s. |
n.s. |
n.s. |
| Rutin |
3.69 |
n.s. |
n.s. |
n.s. |
| Phloretin-C-diglycoside |
14.97 |
n.s. |
0.06 |
n.s. |
| Chlorogenic acid |
15.96 |
0.06 |
0.12 |
n.s. |
| Isosakuranetin |
n.s. |
16.42 |
n.s. |
n.s. |
| Qucercetin |
n.s. |
16.03 |
0.14 |
0.12 |
| Neohesperidin |
n.s. |
1.31 |
0.14 |
n.s. |
| Protocatechuic acid |
n.s. |
0.09 |
n.s. |
n.s. |
| Caffeic acid hexose |
n.s. |
0.06 |
0.20 |
15.90 |
| Naringenin |
n.s. |
n.s. |
15.31 |
n.s. |
| Caffeic acid |
n.s. |
n.s. |
0.09 |
n.s. |
| Hydroxylated naringenin-hexose |
n.s. |
n.s. |
0.10 |
n.s. |
| Sinensetin |
n.s. |
n.s. |
0.08 |
n.s. |
| p-Coumaroyl quinic acid |
n.s. |
n.s. |
0.07 |
n.s. |
| Feruloylquinic acid |
n.s. |
n.s. |
0.06 |
n.s. |
| Pantothenic acid -hexose |
n.s. |
n.s. |
0.06 |
n.s. |
| Quercetin-O-dihexoside |
0.08 |
n.s. |
1.13 |
0.46 |
| Cinnamic acid |
0.11 |
n.s. |
n.s. |
n.s. |
| Eriodictyol-O-dihexoside |
0.06 |
n.s. |
n.s. |
0.06 |
| Ferulic acid |
0.42 |
n.s. |
0.09 |
n.s. |
| Sinapic acid |
0.05 |
n.s. |
0.07 |
n.s. |
| Jasmonic acid | 0.07 | n.s. | 0.06 | n.s. |
Samples at 2 h and 1, 6 and 32 DAT were used for the differential secondary metabolic profiling analysis using the HPLC-QTOF-MS instrument. Four replicates were compared and statistically analysed per sample using Student’s t-test; P < 0.05. After MS/MS analysis, a total of 58 metabolites were found to be significantly regulated (2-fold) in heat treated pericarp when compared to control pericarp (during the same period of storage), and 27 of these metabolites were identified. This table shows the ratio (HT/control during the same period of storage). n.s.: not significant.