Figure 1.

Design of the expression plasmids. All plasmids carry a T7 promoter/terminator, a 6HIS tag for nickel affinity purification, a Tobacco Etch Virus (TEV) cleavage site and a gene encoding a disulfide-rich protein (DRP). The 6HIS tag is N-terminal for the 6HIS plasmid (A) and for fusions without redox properties (B). The 6HIS tag is placed between the fusion partner and the TEV site for fusion with redox properties (C). Non-redox fusion partners are represented in white boxes while redox fusions are represented in blue. The same representation of the redox properties of the fusions appears throughout the figures.