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. 2013 May 7;12:45. doi: 10.1186/1475-2859-12-45

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Copyright © 2013 Kawai et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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pHCA production, EG activity and the cell growth of RsTAL and EG expressing S. lividans. (A) Time-course of pHCA production in culture: S. lividans/pT09pURsTAL cultured in modified TSB medium containing 1% glucose (closed circles), 1% cellobiose (closed squares), or 1% phosphoric acid swollen cellulose (PASC) (closed diamonds); S. lividans/pURsTAL cultured in modified TSB medium containing 1% PASC (closed triangles). (B) Change in culture supernatant endoglucanase activity over time: S. lividans/pT09pURsTAL cultured in modified TSB medium containing 1% glucose (closed circles), 1% cellobiose (closed squares), or 1% PASC (closed diamonds); S. lividans/pURsTAL cultured in modified TSB medium containing 1% PASC (closed triangles). (C) Change in the dry cell weight over time of S. lividans/pT09pURsTAL cultured in modified TSB medium containing 1% glucose (open circles) or 1% cellobiose (open squares). Each data point shows the average of three independent experiments, and error bars represent the standard deviation.