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Generation of Tn5 transposon vector pTN-HYMX for random mutagenesis of Z. mobilis. The xylA/xylB/talB/tktA operon and sHSP/metB/yfdZ operons were cloned into the SalI site of pTN to create the plasmid pTN-HYMX. This plasmid contains SfiI recognized sequences flanking the mosaic end (ME) sites, which are specifically recognized by the EZ-Tn5 transposase.
