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. 2013 May 2;12:41. doi: 10.1186/1475-2859-12-41

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Copyright © 2013 Zhang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Constructions of Tn5 transposon - pTN-HMY. Commercial EZ-Tn5 pMOD vector was mutated to create vector pTN by substituting the PvuI and PshAI with SfiI restriction site. To allow selection of Z. mobilis transformants after electroporation with the Tn5 transposon, tetracycline resistance determinant (TC) was cloned into the multiple cloning site in the vector pTN. The sHSP/metB/yfdZ operon was cloned into the SalI site of pTN to create pTN-HYM. This plasmid contains SfiI restriction site recognized sequences flanking the mosaic end (ME) sites, which are specifically recognized by the EZ-Tn5 transposase.