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Immunohistochemical localization of androgenic enzymes (P450c17, 3βHSD) and accessory protein (cytb5) in ovarian follicles
| Stain | Primordial/primary | Early secondary (<150 μm) | Advanced secondary (>150 μm)
|
Antral
|
Early atretic
|
Advanced atretic
|
Secondary interstitial cells | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| GC | TC | GC | TC | GC | TC | GC | TC | ||||
| P450c17 | − | − | − | + | − | ++ | − | ++ | − | + | + |
| Cytb5 | − | − | − | ++ | − | +++ | − | +++ | − | ++ | ++ |
| 3βHSD | − | − | − | − | − | + | − | +/− | − | +/− | − |
GC, granulosa cells; TC, theca interna cells
