Table 3. Immunoassays available for the detection of PF4/H Abs.
Assay | Vendor | Polyclonal v. IgG Specific |
Principle | Sensitivity | Specificity |
---|---|---|---|---|---|
Asserachrom IgGAM | Diagnostica Stago | Polyclonal | ELISA | 100%71,89 | 64%-86.46%71,89 |
GTI PF4 IgG | GTI Diagnostics | IgG specific | ELISA | 100%89,123 | 42%-95.92%89,123 |
HemosIL AcuStar HIT- Ab(PF4-H) |
Instrumentation laboratory |
Polyclonal | Latex enhanced immunoturbidi metric assay |
100%124 | 81.2%124 |
HemosIL AcuStar HIT- IgG(PF4-H) |
Instrumentation laboratory |
IgG specific | Latex enhanced immunoturbidi metric assay |
100%124 | 96.5%124 |
ID-heparin/PF4 PaGIA |
Diamed | Polyclonal | particle gel immunoassay |
94%-100%71,72,125 | 61%-95%71,72,125 |
Poly-ELISA | GTI Diagnostics | Polyclonal | ELISA | 100%72 | 80.8%72 |
Zymutest HIA IgG | Hyphen Biomed Research |
IgG specific | ELISA | 100%89,123 | 44%-95.92%89,123 |
Zymutest HIA IgGAM | Hyphen Biomed Research |
Polyclonal | ELISA | 100%89 | 87.50%89 |
Enzyme linked immunoassay, ELISA