Figure 3. Non-invasive observation of neutrophils flowing in the blood vessel.

After the injection of 120 μl of PBS containing 330 nM QDs and 670 nM anti-Ly6G antibody, a blood vessel in the mouse auricle 50 μm below the skin surface was observed following injection of QD-antibody complexes in the absence of inflammation. Transillumination images and fluorescent images were captured (30 msec/frame) at the same position. The transillumination image (upper panel) was averaged over successive ten images. Fluorescent images (other panels) were not averaged. A neutrophil flowing in the vessel is indicated by white arrowheads. The bar indicates 10 μm. A movie of the fluorescent image is available in Supplementary Movie 1.