FIGURE 2. Liver injury from GalN/LPS is unaffected by loss of jnk1.

A, total hepatic protein was isolated from wild-type (+/+) or jnk1^−/− (−/−) mice at the indicated hours after treatment with GalN/LPS and immunoblotted with phospho-JNK (P-JNK), total JNK, caspase-3 and -7, PARP, and β-actin antibodies. The p54 and p46 JNK bands are labeled, and the caspase-3 and -7 and PARP cleavage products are indicated by arrows. Findings are representative of three independent experiments. B, serum ALT levels from wild-type (WT) and jnk1^−/− mice at 4 and 6 h after GalN/LPS administration. C, graded histological scoring of the degree of apoptosis (Apop) and inflammation (Inflam) in the same livers. D, numbers of TUNEL positive cells per high power field in the identical liver sections. Data in B–D are from three independent experiments and total five–eight animals per data point.