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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Mar 18;1830(6):3399–3406. doi: 10.1016/j.bbagen.2013.03.010

Figure 1. Selenium supplementation affects pH2AX levels and cell viability in HCT116 and NCM460 cells exposed to radiation.

Figure 1

HCT116 (A) and NCM460 (B) cells were pre-treated with or without additional 30nM sodium selenite for 3 days and exposed to 4Gy of ionizing radiation and after 1hr, pH2AX levels were measured by flow cytometry. HCT 116 (C) and NCM460 (D) cells were either untreated or treated with 30nM NaSe and exposed to 0, 1, 3, 5 Gy ionizing radiation, replated into 96 well plates and MTT assays were performed on day 1 and 5. *P<0.05, Ψ P<0.005 and § P<0.0005 from No IR control. Error bars indicate the S.D.