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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Mar 18;1830(6):3399–3406. doi: 10.1016/j.bbagen.2013.03.010

Figure 2. Selenium pre-treatment reduces bleomycin induced pH2AX fluorescence in HCT116 and NCM460 cells but does not affect cell viability.

Figure 2

HCT116 (A) and NCM460 (B) were either untreated or treated with 30nM selenium for 3 days and exposed to 10μg/ml bleomycin for 1hr; cells were fixed and stained for pH2AX. The graphs represent the mean fluorescent intensity of the cells stained for pH2AX as determined by flow cytometry. HCT116 (C) and NCM460 (D) cells were pretreated with or without a supplemental 30 nM selenium for 3 days before treating with 10μg/ml bleomycin. MTT assays were performed at 48 hrs post-bleomycin treatment. § P<0.0005 from Control, ¥ P<0.05 form bleomycin. Error bars indicate the S.D.