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. 2013 Apr 4;108(8):1648–1658. doi: 10.1038/bjc.2013.122

Figure 5.

Figure 5

Effect of miR-874 on HDAC1 mRNA and protein expression in SAS and FaDu cells. (A) HDAC1 mRNA expression 72 h after transfection with 10 nℳ miR-874. HDAC1 mRNA expression was significantly repressed in miR-874 transfectants. GAPDH was used as an internal control. (B) HDAC1 protein expression 72 h after transfection with miR-874. GAPDH was used as a loading control. The protein expression level of HDAC1 was also repressed in miR-874 transfectants. (C) Interaction of miR-874 with HDAC1 3′-UTR. After 24-h transfection with 10 nℳ miR-874, miR-control or mocks, a reporter plasmid containing HDAC1 wild type-3′-UTR or deletion-3′-UTR and a plasmid expressing Renilla luciferase (hRluc) were co-transfected into SAS cells. Firefly luciferase activity was normalised to Renilla luciferase activity. Relative luciferase activity in miR-874 transfectants was compared with that in mock cultures, which was set at 1, in cells transfected with wild type-3′-UTR or deletion-3′-UTR. Trials were independently conducted three times. *P<0.01.