Figure 3.

Effect of the combination IMO and everolimus on ACHN or 786-O RCC tumour xenografts in nude mice.

After 7 days from s.c. injection of ACHN or 786-O cells, mice were randomised (six per group) to receive IMO, everolimus or their combination, as described in the Materials and Methods section. The one-way ANOVA test was used to compare tumour sizes among different treatment groups at the median survival time of the control group (31 days). They were statistically significant for IMO, everolimus and the combination vs control (P<0.0001). Bars, s.d. (A for ACHN, D for 786-O). Median survival was statistically significant for IMO, everolimus and their combination vs control (log-rank test; B for ACHN, E for 786-O). Western blot analysis was performed on total lysates from ACHN tumour specimens of two mice treated as described in the Materials and Methods section and killed on day 25 (C for ACHN, F for 786-O).