|
KRAS codon
12 (outer)
|
3F |
5′-ACTGAATATAAACTTGTGGTAGTTGGACCT–3′ |
95 °C × 10 min; (95 °C × 30 s, 50 °C × 1 min, 72 °C × 1 min) × 20 cycles; 72 °C × 3 min |
143 |
|
10B
|
5′-ACTCATGAAAATGGTCAGAGAAACCTTTAT-3′
|
|
|
| KRAS codon 13 (outer) |
9F |
5′-ACTGAATATAAACTTGTGGTAGTTGGCCCTGGT-3′ |
95 °C × 10 min; (95 °C × 30 s, 54 °C × 1 min, 72 °C × 1 min) × 20 cycles; 72 °C × 3 min |
113 |
|
10B
|
5′-ACTCATGAAAATGGTCAGAGAAACCTTTAT-3′
|
|
|
| KRAS codon 12 (inner) |
3F |
5′-ACTGAATATAAACTTGTGGTAGTTGGACCT-3′ |
95 °C × 10 min; (95 °C × 30 s, 54 °C × 1 min, 72 °C × 1 min) × 45 cycles; 72 °C × 3 min |
143 |
| |
14B |
5′-TCAAAGAATGGTCCTGGACC-3′ |
|
|
| KRAS codon 13 (inner) |
9F |
5′-ACTGAATATAAACTTGTGGTAGTTGGCCCTGGT-3′ |
|
113 |
|
4B
|
5′-TCAAAGAATGGTCCTGCACC-3′
|
|
|
| EGFR exon 18 |
EGFR18F |
5′-TCCAGCATGGTGAGGGCTGAG-3′ |
50 °C × 2 min; 95 °C × 10 min; (95 °C × 40 s, 58 °C × 40 s, 72 °C × 35 s) × 40 cycles; 72 °C × 3 min |
242 |
| |
EGFR18R |
5′-GGCTCCCCACCAGACCATG-3′ |
|
|
| EGFR exon 19 |
EGFR19F |
5′-TGGGCAGCATGTGGCACCATC-3′ |
|
217 |
| |
EGFR19R |
5′-AGGTGGGCCTGAGGTTCAG-3′ |
|
|
| EGFR exon 20 |
EGFR20F |
5′-CCTCCTTCTGGCCACCATGCG-3′ |
|
296 |
| |
EGFR20R |
5′-CATGTGAGGATCCTGGCTCC-3′ |
|
|
| EGFR exon 21 |
EGFR21F |
5′-CCTCACAGCAGGGTCTTCTC-3′ |
|
229 |
|
EGFR21R
|
5′-CCTGGTGTCAGGAAAATGCT-3′
|
|
|
| BRAF exon 15 |
BRAF15F |
5′-TCATAATGCTTGCTCTGATAGGA-3′ |
95°C × 10 min; (95 °C × 15 s, 52 °C × 30 s, 72 °C × 30 s) × 45 cycles; 72 °C × 3 min |
224 |
|
BRAF15R
|
5′-GGCCAAAAATTTAATCAGTGGA-3′
|
|
|
| PIK3CA exon 9 |
PIK3CA9F |
5′-GGGAAAAATATGACAAAGAAAGC-3′ |
95 °C × 10 min; (95 °C × 35 s, |
251 |
| |
PIK3CA9R |
5′-CTGAGATCAGCCAAATTCAGTT-3′ |
56 °C × 30 s, 72 °C × 30 s) × 40 cycles; 72 °C × 10 min |
|
| PIK3CA exon 20 |
PIK3CA20FPIK3CA20R |
5′-CTCAATGATGCTTGGCTCTG-3′5′-TGGAATCCAGAGTGAGCTTTC-3′ |
|
241 |
