Skip to main content
. 2013 Mar 28;108(8):1634–1640. doi: 10.1038/bjc.2013.124

Figure 1.

Figure 1

The CXCR4/CXCL12 axis in melanoma migration to the liver. (A) Representative example of immunofluorescence staining of human liver tissue from a patient with melanoma metastasis stained for α-SMA (green) and MelanA (red). Original magnification, × 20; scale bar=42 μℳ. Image is representative of five patients. (B) Representative example of immunofluorescence staining of primary myofibroblasts (passage 3). Cells were incubated (i) without primary antibody or (ii) with antibody to α-SMA (green) or (iii) with anti-CXCL12 (red). (iv) Overlay of each image with yellow staining indicating costaining of α-SMA and CXCL12. Cells were counterstained with DAPI (4',6-diamidino-2-phenylindole) to visualise the nuclei and images were taken by confocal microscopy (original magnification, × 20; scale bar=150 μℳ). Image is representative of three patients. (C) Representative example of double immunofluorescence staining of metastatic melanoma sections demonstrating staining with (i) antibody to CXCR4 (green) or (ii) anti-MelanA (red). (iii) Overlay of each image with yellow staining indicating costaining of MelanA and CXCR4. (iv) Cytofluorogram scatterplot of CXCR4 and MelanA staining. Cells were counterstained with DAPI to visualise the nuclei and images were taken by confocal microscopy (original magnification, × 20; scale bar=150 μℳ). Image is representative of five patients.