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(A) Effect of B-RAF-V600E on melanoma migration. Western blotting for B-RAF in melanoma cell lines with densitometric quantification. (B) Western blotting as indicated in CHL-1 transiently transfected with WT or V600E B-RAF. (C) CHL-1 cells transfected with either WT (black bars) or mutant (V600E) (grey bars) B-RAF migrated for 16 h towards 10 nℳ CXCL12 across an 8-μm pore size filter in the presence of 6.6 μℳ AMD11070. HPF, high-power field.
