FIG. 2.

Expression of endodermal marker proteins in Wnt3a/BMP4-treated cells. (A) Immunostaining of differentiated cells for β-catenin (top panels, insets show β-catenin only, arrowheads indicate nuclear localization of β-catenin), Pax6/Nestin (middle panels, inset show Pax6 only) and Sox17/E-cadherin (bottom panels) in different culture conditions. Scale bars: 20 μm. (B) Flow cytometric analysis of Nestin, Sox17, Foxa2, and GATA4-expressing cells in control and Wnt3a/BMP4 conditions. Data shown are representative histograms from three independent experiments. The percentage of positive cells was indicated. Red, isotype control. Color images available online at www.liebertpub.com/scd