Table 3.

Primer Sequences for mESC Pluripotency Gene Profile by Reverse Transcriptase Polymerase Chain Reaction

Serial No.	Gene accession no.	Gene symbol	Primer sequences (forward & reverse)
1	NT_166349.1	GAPDH	5′-TCAACAGCAACTCCCACTCTTCCA-3′ 5′-ACCCTGTTGCTGTAGCCGTATTCA-3′
2	NM_013633.2	Oct-4	5′-GCTCTCCCATGCATTCAAAC-3′ 5′-TGTCTACCTCCCTTGCCTTG-3′
3	NM_011443	Sox-2	5′-ATGGCCCAGCACTACCAGAG-3′ 5′-CTTCTCCAGTTCGCAGTCCA-3′
4	NM_028016.2	Nanog	5′-TTTGGAAGCCACTAGGGAAAG-3′ 5′-AAGCCCAGATGTTGCGTAAGT-3′
5	NM_011920	ABCG2	5′-CCATAGCCACAGGCCAAAGT-3′ 5′-GGGCCACATGATTCTTCCAC-3′
6	NM_006086	β-III Tubulin	5′-GGAACATAGCCGTAAACTGC-3′ 5′-TCACTGTGCCTGAACTAACTTACC-3′
7	NM_144841	Otx2	5′-GCTGGCTCAACTTCCTACT-3′ 5′-TCCAAGCAGTCAGCATTGAAG-3′
8	NM_008090.5	GATA-2	5′-GCAGAGAAGCAAGGCTCGC-3′ 5′-CAGTTGACACACTCCCGGC-3′
9	NM_003181	Brachyury (T)	5′-ATAACGCCAGCCCACCTACT-3′ 5′-TGGTACCATTGCTCACAGACC-3′
10	NM_008092	GATA-4	5′-TCAAATTCCTGCTCGGACTTGGGA-3′ 5′-GTTTGAACAACCCGGAACACCCAT-3′
11	NM_008814	PDX-1	5′-ACTTAACCTAGGCGTCGCACAAGA-3′ 5′-GGCATCAGAAGCAGCCTCAAAGTT-3′