Fig. 3.

Pattern of MyoD and Sulf1A expression in satellite cells on dissociated single muscle fibres grown in the presence of only Wnt6 secreting cells as a control (lane 1) and in the presence of 100 ng/ml (lanes 2–4 representing three different experiments to demonstrate reproducibility) or 300 ng/ml (lane 5) Sulf1A peptide antibodies for 72 h investigated by double immunofluorescence procedure. Sulf1A inhibition by neutralising antibodies clearly apparent from reduced Sulf1A expression in a large majority of the satellite cells, reverses Wnt6-induced satellite cell differentiation and proliferation inhibition. A further increase in the Sulf1 antibody concentration triggering initial proliferation appeared to lead to some apoptosis.