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. 2013 Jan 22;3:83–88. doi: 10.1016/j.fob.2013.01.004

Fig. 4.

Fig. 4

Endospanin-2 is expressed in rat osteoclasts. Total RNA was isolated from rat osteoclasts enriched by magnetic separation with a β3-integrin antibody from newborn rat bone marrow (Osteoclasts) and the remaining cell population (Non-Osteoclasts). After reverse transcription, Endospanin-2, Cathepsin K and GAPDH were amplified with gene specific primer pairs by PCR.