Fig. 4.
Relative populations of SS intermediates of (A) CX-397, (B) Ins-A, (C) Rlx-A, and (D) Ins-B at 25 °C as a function of the reaction time. Reaction conditions: (A) pH = 8.0 (100 mM Tris–HCl buffer), [RCX-397]0 = [DHSox]0/2 = 53.1 μM; (B) pH = 8.0 (100 mM Tris–HCl buffer with 2 M urea), [RIns-A]0 = [DHSox]0 = 23.3 μM; (C) pH = 7.0 (100 mM Tris–HCl buffer with 2 M urea), [RRlx-A]0 = [DHSox]0 = 30.7 μM; and (D) pH = 8.0 (100 mM Tris–HCl buffer with 2 M urea), [RIns-B]0 = [DHSox]0 = 31.0 μM. Curves were drawn by simulation using the second-order rate constants obtained by fitting the experimental data to reaction schemes of Eqs. (2)–(5).