Fig. 1.

S897A mutation abolishes ligand-independent promotion of cell migration by EphA2 in MCF-7 and HeLa cells. (A and B) Motility of MCF-7 (A) or HeLa cells (B) was evaluated by Transwell cell migration assays. Cells transfected with YFP alone or together with EphA2-WT or EphA2-SA were plated in the upper chamber of the filters, and at 4 h after plating, cells that had migrated to the underside of the filters were fixed. Relative cell migration was determined by the number of the YFP-positive migrated cells normalized to the total number of the YFP-positive attached cells. Data are presented as the means ± SEM from four or five independent experiments (***, P < 0.001; t-test). (C) The cell lysates of HeLa cells transfected with Myc-EphA2-WT or -SA were immunoprecipitated with ephrinA1-Fc, and bound proteins and total cell lysates were analyzed by immunoblotting with the indicated antibodies.