Fig. 3.

S897A mutation abolishes EphA2-induced RhoG activation. Cell lysates from HeLa cells co-transfected with Myc-RhoG and Myc-EphA2-WT or EphA2-SA were incubated with GST-ELMO, and bound Myc-RhoG (Pull-down) and total cell lysates (Lysates) were analyzed with anti-Myc antibody. Relative RhoG activity was determined by the amount of GTP-bound active Myc-RhoG normalized to the amount of Myc-RhoG in cell lysates analyzed by ImageJ software, and the data shown are relative to the basal activity (None). Data are presented as the means ± SEM from three independent experiments (*, P < 0.05; t-test).