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. 2013 Jan 21;3:78–82. doi: 10.1016/j.fob.2013.01.002

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© 2013 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig. 4 — Phosphorylation of EphA2 on S897 regulates the interaction between EphA2 and Ephexin4. (A–C and E) The cell lysates of HEK293T cells transfected indicated plasmids were immunoprecipitated with ephrinA1-Fc, and bound proteins and total cell lysates were analyzed by immunoblotting with the indicated antibodies (p-Akt-Sub, an antibody that recognized phosphorylated Akt substrates at Akt consensus phosphorylation sequence; myr-Akt, Akt fused with the Src myristoylation signal at the NH2 terminus). (D) The lysates of MCF-7 cells were immunoprecipitated with anti-EphA2 antibody, and bound proteins and total cell lysates were analyzed by immunoblotting with the indicated antibodies (pS897-EphA2, an antibody against phosphorylated S897 of EphA2).